4/23/2024 0 Comments Prey vs predator lab![]() A different prey community was found outside the reserve, which resulted in different prey composition for otters. Using 12S primers and a 5% threshold, we found no seasonal variation of otter diet in the Tangjiahe National Nature Reserve. Both 12S and 16S primers revealed similar otter diets, indicating that a single set of primers with a higher threshold is cost-effective for detecting the main prey taxa. ![]() Diet did not vary among different data removal thresholds of 0.1, 1, 3, and 5%, comprising fishes (>90%), amphibians and birds (>2%), and occasionally mammals (<2%). We applied DNA metabarcoding to analyzing the diet of the Eurasian otter ( Lutra lutra), a top predator in freshwater ecosystems, using 12S and 16S rRNA mitochondrial primers target vertebrate prey. While the morphological identification of scat contents is the traditional method, DNA metabarcoding has lately proven a more efficient and accurate method of identifying prey taxa. However, the elusive habit and low population density of many predators constrains determination of their diets. The diet of top predators is vital information needed to determine their ecological function and for their conservation management. 3Tangjiahe National Nature Reserve, Guangyuan, China.2School of Life Sciences, Sun Yat-sen University, Guangzhou, China.1State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.Qiaoyun Wang 1 Zichen Wang 2 Kaidan Zheng 1 Peng Zhang 2 Limin Shen 3 Wanli Chen 3 Pengfei Fan 1* Lu Zhang 1* ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |